Plasmid

Part:BBa_K5048057

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid pBAD lasR luxl

This plasmid uses pBAD as backbone. Upon induction with arabinose, the bacteria will express lasR. On this basis, with the addition of lasI, the bacteria will express the ccdB toxin and die.On top of that, it will synthesis luxl.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2044
    Illegal BglII site found at 2020
    Illegal BamHI site found at 2010
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1782
    Illegal BsaI site found at 2223
    Illegal BsaI site found at 3290
    Illegal SapI.rc site found at 4372


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Categories
Parameters
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